Action of Lysolecithin on Blood Platelets

Chava Kirschmann; Sara Aloof; Andre de Vries

doi:10.1055/s-0038-1655005

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1963; 09(03): 512-524
DOI: 10.1055/s-0038-1655005

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Action of Lysolecithin on Blood Platelets^[*]

Chava Kirschmann

¹Rogoff Medical Research Institute, Department of Experimental Biology of the Tel Aviv University and the Labor Sick fund, Beilinson Hospital, Petah Tikva, Israel

,

Sara Aloof

¹Rogoff Medical Research Institute, Department of Experimental Biology of the Tel Aviv University and the Labor Sick fund, Beilinson Hospital, Petah Tikva, Israel

,

Andre de Vries

¹Rogoff Medical Research Institute, Department of Experimental Biology of the Tel Aviv University and the Labor Sick fund, Beilinson Hospital, Petah Tikva, Israel

› Author Affiliations

Abstract

Summary

Lysolecithin is adsorbed to washed blood platelets and, at sufficient concentration, lyses them, inhibits their clot-retracting activity and promotes their thromboplastin-generating activity. Lysolecithin adsorption to the platelet was studied by using P³²-labelled lysolecithin obtained from the liver of rats injected with labelled orthophosphate. The amount of lysolecithin adsorbed to the surface of the washed platelet in saline medium is dependent on the concentration of lysolecithin in solution and reaches saturation — 5 × 10^-8 jig per platelet — at a concentration of 9—10 µg per ml. Platelet lysis in saline medium begins at a lysolecithin concentration higher than 18 jig per ml. Plasma and albumin prevent adsorption of lysolecithin to the platelet and protect the platelet from damage by lysolecithin. Albumin is able to remove previously adsorbed lysolecithin from the platelet surface. The protective action of plasma explains the lack of platelet damage in blood, the plasma lecithin of which has been converted to lysolecithin by the action of Vipera palestinae venom phosphatidase, in vitro and in vivo.

Full Text

References

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Action of Lysolecithin on Blood Platelets[*]

Action of Lysolecithin on Blood Platelets^[*]